The objectives of this study were to explore its vascular and antioxidant properties on isolated rat aorta and cell free antioxidant systems. Methods: Plant material: Fresh flowers were collected in the forest surrounding Botanical Garden of Central University of Las Villas. Phytochemical procedure: Etanolic (EF), ethylacetate (EAF) and butanolic (BF) fractions from flowers were obtained by soxhlet apparatus method with EtOH. The initial crude ethanolic extract was subject to sequential liquid-liquid extraction procedure with n-Hexane, ethylacetate and n-butanol in order to obtain the respective fraction. The antioxidant properties on cell-free system were assessed through DPPH, Reducting power, phosphomolybdenum method and metal chelating tests. Isolated tissue studies: Endothelium-intact aortic rings, 4 mm, were used to test the vascular effects of EAF. KCl (30 mM) was used for check tissue viability. Phenylephrine (0.5 μM) and KCl (30 mM) were used for elicited contraction (independent experiments) and after EAF was added (10-400 μg/ml) in cumulative manner to the organ bath. Isoprenaline and verapamil, according to the specific test, were used as references. Vascular toxicity test: Aortic rings were incubated 1 h under EAF (1000 μg/ml) or vehicle, washed several times and then the contractile response to KCl (30 mM) was tested. Results: The EAF showed the best antioxidants properties and then was selected for vascular studies. The EAF exert a dual effect (relaxation-contraction) on phenylephrine (0.5 μM) and KCl (30 mM) stimulated aortas. Any toxicity effect over vascular samples was observed. Conclusions: This study revealed the antioxidant and vascular properties of EAF obtained from Kigelia africana flowers.