VII Simposio Internacional de Química 2019 "SIQ 2019" -VII Conferencia "Ciencias Químicas"

VII Simposio Internacional de Química 2019

SIQ 2019

Proteomic Characterization of Outer Membrane Vesicle Vaccine From Vibrio cholerae.

Cholera epidemics affect a large number of African, Asian and Caribbean countries. The climatic changes and the constant migrations cause that this disease spreads so, it is necessary to obtain protective vaccines. In the present work, a new vaccine of outer membrane vesicles (OMV) from V. cholerae O1 biotype El Tor serotype Ogawa strain C7258 at Finlay Institute of vaccines (Cuba) was characterized by proteomic methods. We identified 53 proteins present in the OMV (approximate ratio of 4 proteins by electrophoresis band) separated by one dimension electrophoresis and digested by tripsin method. The fragments were separated by high pressure chromatography (HPLC) coupled to mass spectrometry, sequenced and identified using Swiss_Prot and TrEMBL protein databases for V. cholera genome. The pattern presented some of proteins (12 cytoplasm proteins and 5 outer membrane proteins) suggested within the OMV highest quality proteome for vaccine candidate. The best conditions for proteins separation by two dimension electrophoresis allowed the resolution of 241 spots, increasing the size of focusing strips (to 24 cm), application in gel and a focusing time of 60 KVh. The OMV composition was based on proteins described to the immunity response and protection against V. cholera O1 biotype El Tor serotype Ogawa.

Cholera epidemics affect a large number of African, Asian and Caribbean countries. The climatic changes and the constant migrations cause that this disease spreads so, it is necessary to obtain protective vaccines. In the present work, a new vaccine of outer membrane vesicles (OMV) from V. cholerae O1 biotype El Tor serotype Ogawa strain C7258 at Finlay Institute of vaccines (Cuba) was characterized by proteomic methods. We identified 53 proteins present in the OMV (approximate ratio of 4 proteins by electrophoresis band) separated by one dimension electrophoresis and digested by tripsin method. The fragments were separated by high pressure chromatography (HPLC) coupled to mass spectrometry, sequenced and identified using Swiss_Prot and TrEMBL protein databases for V. cholera genome. The pattern presented some of proteins (12 cytoplasm proteins and 5 outer membrane proteins) suggested within the OMV highest quality proteome for vaccine candidate. The best conditions for proteins separation by two dimension electrophoresis allowed the resolution of 241 spots, increasing the size of focusing strips (to 24 cm), application in gel and a focusing time of 60 KVh. The OMV composition was based on proteins described to the immunity response and protection against V. cholera O1 biotype El Tor serotype Ogawa.

Sobre el ponente

Maria de Los Angeles  Padron Collazo

MsC. Maria de Los Angeles Padron Collazo

Instituto Finlay de Vacunas Flag of Cuba
Información Práctica
English (US)
No definido
30 minutos
No definido
Autores
Rubén A Cabrera
Sonia Nisar
Ernesto Milián
Bárbara Cedré
Mildrey Fariñas
Gemma Año
Tania Valmaseda
Luis Riverón
Yisabel Aranguren
Arturo Talavera
Daily Serrano
Yamisley González
Hilda Garcia
Michel Acosta
Luis García
Ileana Martínez
Jun X Wheeler
Christopher Jones
Maydelis Alvarez
MsC. Maria de Los Angeles Padron Collazo
José L Pérez
Gustavo V Sierra
Palabras clave
outer membrane proteins
proteomic methods
vaccines
vibrio cholerae
Documentos