The aim of the study was to determine the seroprevalence of Toxoplasma gondii infection in domestic pigs in Villa Clara, central region of Cuba, and to evaluate some risk factors associated with the infection. Serum samples from 420 (210 sows, 210 pre-fattening pigs) pigs, from the seven porcine breeding farms of the province were tested for antibodies against T. gondii by indirect enzyme-linked immunosorbent assay (iELISA). Antibodies to T. gondii were detected in 66 pigs of 420 pigs tested (15.71%, 8.10% in pre-fattening pigs and 23.3% in sows). The herd prevalence was 100% and significant differences were evident within-Farm prevalence (Chi2=20.35, P<0.01) which ranged from 6.67% to 31.67%. A generalized estimating equations model indicated that the risk factors associated with T. gondii seroprevalence were: age, sows compared to pre-fattening pigs (OR=3.567; 95% CI=1.979–6.463) and altitude (OR=2.565; 95% CI=1.469–4.480). This is the first study documenting the seroprevalence of T. gondii infection of pigs in Villa Clara. These results indicated widespread exposure to T. gondii among domestic pigs in Villa Clara, as well as may contribute to the development of optimal preventive measures against T. gondii infection in domestic pig farms to avoid the Toxoplasma gondii infection in humans.