VII Simposio Internacional de Ciencias Farmacéuticas 2019 "VII SICF" -IV Simposio Internacional "Química de los productos naturales"

VII Simposio Internacional de Ciencias Farmacéuticas 2019

VII SICF

Antioxidant activity and phenolic composition of the aqueous extract from seaweed Bryothamnion triquetrum.

Resumen [ES]

Seaweed has been widely studied in recent years as a natural source of antioxidant compounds for the treatment of different pathologies. In this work, the antioxidant activity of the aqueous extract of the Bryothamnion triquetrum seaweed was studied by means of the reducing power assay (FRAP), its protective effects against the hemolysis induced by AAPH and the determination of the cell viability against the H2O2-induced oxidative stress in the H-125 cell line by using MTT and sulforrodamine. The aqueous extract was able to reduce the ferrous species to ferrous with a dose-dependent response, protect against AAPH-induced hemolysis in sheep erytrocytes and was maintained cell viability above 80% of the total cells at concentrations of seaweed higher than 800 µg / mL. The estimation of the total polyphenol content revealed that this seaweed presents 47.81 ± 2.35 μg EAG / mg of lyophilized extract. The extract methanol: H2O (1:1) present 11 polyphenolic acids, the afzelequina, cumaric and ascorbic acid, which justifies the antioxidant activity reported in this work.

Resumen [EN]

Seaweed has been widely studied in recent years as a natural source of antioxidant compounds for the treatment of different pathologies. In this work, the antioxidant activity of the aqueous extract of the Bryothamnion triquetrum seaweed was studied by means of the reducing power assay (FRAP), its protective effects against the hemolysis induced by AAPH and the determination of the cell viability against the H2O2-induced oxidative stress in the H-125 cell line by using MTT and sulforrodamine. The aqueous extract was able to reduce the ferrous species to ferrous with a dose-dependent response, protect against AAPH-induced hemolysis in sheep erytrocytes and was maintained cell viability above 80% of the total cells at concentrations of seaweed higher than 800 µg / mL. The estimation of the total polyphenol content revealed that this seaweed presents 47.81 ± 2.35 μg EAG / mg of lyophilized extract. The extract methanol: H2O (1:1) present 11 polyphenolic acids, the afzelequina, cumaric and ascorbic acid, which justifies the antioxidant activity reported in this work.

Sobre el ponente

Jessica Gómez Lemus

Lic. Jessica Gómez Lemus

Facultad de Biología, Universidad de La Habana Flag of Cuba
Información Práctica
Póster digital
Spanish / Español
junio 26, 2019 2:46 p. m.
5 minutos
Restaurante "El Tesico"
Autores
Alexis Vidal
Iraida Spengler
Daylín Díaz Gutierrez
Lic. Jessica Gómez Lemus
Trina García
Alina Forrellat
Palabras clave
antioxidant activity
bryothamnion triquetrum
phenolic composition