VII Simposio Internacional de Ciencias Farmacéuticas 2019 "VII SICF" -IV Simposio Internacional "Química de los productos naturales"

VII Simposio Internacional de Ciencias Farmacéuticas 2019

VII SICF

IV Simposio Internacional "Química de los productos naturales"

OPTIMIZATION OF FLAVONOIDS EXTRACTION FROM ANNONA MURICATA L. LEAVES

Resumen [ES]

Introduction: Annona muricata L. (soursop) is used in ethnomedicine for the treatment of various diseases. Leaves of this species have been found to contain flavonoids, which are compounds with antidiabetic, antioxidant and anti-inflammatory properties. Objective: optimization of operational parameters on the solid–liquid extraction of flavonoids from A. muricata leaves. Methods: Evaluation of the operational variables was based on Composite Center Design (CCD) of Response surface methodology (RSM), selecting the intervals between 50 and 70 ml/g for the plant material / solvent volume ratio, 40% to 80% for ethanol concentration and 2 to 3 h for extraction time. Flavonoids and quercetin were found in the extracts obtained. Total flavonoids were quantified using a colorimetric method at 430 nm, expressed as quercetin. Experiments were conducted in triplicate to determine the best experimental condition. Comparison with the design value predicted showed a good correlation between the two. Results: the optimal condition for extraction of total flavonoids was reached at an ethanol concentration of 96%, a 70 ml/g ratio and a time of 1.6 h. The variables found to be significant were the quadratic effects of the plant material / solvent volume ratio and ethanol concentration. Conclusions: a selection was made of the best conditions to obtain an extract from soursop leaves containing flavonoids, in which an extraction output of 87% was achieved in the extraction process.

Resumen [EN]

Introduction: Annona muricata L. (soursop) is used in ethnomedicine for the treatment of various diseases. Leaves of this species have been found to contain flavonoids, which are compounds with antidiabetic, antioxidant and anti-inflammatory properties. Objective: optimization of operational parameters on the solid–liquid extraction of flavonoids from A. muricata leaves. Methods: Evaluation of the operational variables was based on Composite Center Design (CCD) of Response surface methodology (RSM), selecting the intervals between 50 and 70 ml/g for the plant material / solvent volume ratio, 40% to 80% for ethanol concentration and 2 to 3 h for extraction time. Flavonoids and quercetin were found in the extracts obtained. Total flavonoids were quantified using a colorimetric method at 430 nm, expressed as quercetin. Experiments were conducted in triplicate to determine the best experimental condition. Comparison with the design value predicted showed a good correlation between the two. Results: the optimal condition for extraction of total flavonoids was reached at an ethanol concentration of 96%, a 70 ml/g ratio and a time of 1.6 h. The variables found to be significant were the quadratic effects of the plant material / solvent volume ratio and ethanol concentration. Conclusions: a selection was made of the best conditions to obtain an extract from soursop leaves containing flavonoids, in which an extraction output of 87% was achieved in the extraction process.

Sobre el ponente

Suslebys Salomón Izquierdo

MsC. Suslebys Salomón Izquierdo

CIDEM Flag of Cuba
Información Práctica
Ponencia
English (US)
junio 26, 2019 11:15 a. m.
20 minutos
Restaurante "El Tesico"
Autores
Marilín López Armas
Yaima Henry García
MsC. Suslebys Salomón Izquierdo
Aylema Romero Díaz
Jhoany Acosta Esquijarrosa
Palabras clave
annona muricata l. leaves
flavonoids
response surface methodology
solid–liquid extraction