IX Conferencia Científica Internacional Desarrollo Agropecuario y Sostenibilidad "AGROCENTRO 2019" -IX Simposio de Medicina Veterinaria y Zootecnia

IX Conferencia Científica Internacional Desarrollo Agropecuario y Sostenibilidad

AGROCENTRO 2019

EFFECT OF THE PRESENCE OF CORPORA LUTEA ON IN VITRO EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES

Resumen [ES]

In vitro fertilization (IVF) with conventional and sex-sorted semen is used worldwide. Cleavage and blastulation are essential for a successful pregnancy (Peippo et al., 2001). Previous studies have reported inconsistent results regarding the effect of a corpus luteum (CL) on embryonic development (Islam et al 2007; Hajarian et al, 2016). The objective of this study was to determine the effect of CL on cleavage and blastulation rates of bovine oocytes after IVF with conventional or sex-sorted semen. Ovaries from slaughtered Holstein cows were collected. Those with at least one CL formed CCL group, while ovaries without CL formed OCL group. A total of 747 oocytes were collected and those of categories 1 or 2 (CCL n=238 and OCL n=138; Stojkovic et al. 2001) were matured, fertilized with conventional or sex-sorted semen (CONV n=133, SEX n=243) and cultured in vitro. Proportions of cleavage and blastulation in CCL and OCL groups fertilized by both types of semen were compared in a combined model using SAS (version 9.0) software. No interaction between semen type and corpus luteum criteria were observed. CONV showed higher proportions of cleavage and blastulation (104/133, 78.2% and 24/133, 18.0%, respectively) than SEX (102/243, 42.0% and 23/243, 9.5%, respectively; P<0.05). OCL yielded higher proportions of cleavage and blastulation (99/138, 77.7% and 26/138, 18.9%, respectively) than CCL (107/238, 44.9% and 21/238, 8.8%, respectively; P<0.01). Sex-sorted semen appears to be lees capable of in vitro fertilization, and that the presence of CL affects negatively cleavage and blastulation of bovine oocytes.

Resumen [EN]

In vitro fertilization (IVF) with conventional and sex-sorted semen is used worldwide. Cleavage and blastulation are essential for a successful pregnancy (Peippo et al., 2001). Previous studies have reported inconsistent results regarding the effect of a corpus luteum (CL) on embryonic development (Islam et al 2007; Hajarian et al, 2016). The objective of this study was to determine the effect of CL on cleavage and blastulation rates of bovine oocytes after IVF with conventional or sex-sorted semen. Ovaries from slaughtered Holstein cows were collected. Those with at least one CL formed CCL group, while ovaries without CL formed OCL group. A total of 747 oocytes were collected and those of categories 1 or 2 (CCL n=238 and OCL n=138; Stojkovic et al. 2001) were matured, fertilized with conventional or sex-sorted semen (CONV n=133, SEX n=243) and cultured in vitro. Proportions of cleavage and blastulation in CCL and OCL groups fertilized by both types of semen were compared in a combined model using SAS (version 9.0) software. No interaction between semen type and corpus luteum criteria were observed. CONV showed higher proportions of cleavage and blastulation (104/133, 78.2% and 24/133, 18.0%, respectively) than SEX (102/243, 42.0% and 23/243, 9.5%, respectively; P<0.05). OCL yielded higher proportions of cleavage and blastulation (99/138, 77.7% and 26/138, 18.9%, respectively) than CCL (107/238, 44.9% and 21/238, 8.8%, respectively; P<0.01). Sex-sorted semen appears to be lees capable of in vitro fertilization, and that the presence of CL affects negatively cleavage and blastulation of bovine oocytes.

Sobre el ponente

Andres Quezada Casasola

Andres Quezada Casasola

Universidad Autónoma de Ciudad Juárez Flag of México
Información Práctica
English (US)
No definido
30 minutos
No definido
Autores
Andres Quezada Casasola
M. Ortiz
Roldán Domínguez
D.e. Cano Reagan
A. M. Ávila Escárcega
E. Orozco Lucero
J. M. Carrera Chávez
Palabras clave
blastulation
cleavage
corpora lutea
in vitro fertilization
sexed semen