VII Simposio Internacional de Ciencias Farmacéuticas 2019 "VII SICF" -IV Simposio Internacional "Química de los productos naturales"

VII Simposio Internacional de Ciencias Farmacéuticas 2019

VII SICF

Properties of complex sugars extracted from sugar maple bark

Resumen [ES]

The sugar maple is a native species of northeastern North America and is widely appreciated and exploited for its sap, which is concentrated to produce maple syrup. However, its bark is not widely used, despite its content of a variety of bioactive compounds which could be applied in health products. Apart from polyphenols, higher molecular weight compounds such as oligo- and polysaccharides have been less explored so far. The aim of this investigation was to isolate and characterize a complex sugar from sugar maple bark and evaluate its biological activities. Two samples obtained by extraction with water and ethanol-water (v/v:1/1) at 80°C, have been studied in this research. Sugar components were concentrated by ethanol (95%) precipitation and ultrafiltration (5KDa MWCO) to obtain enriched polysaccharides fractions. The latter were separated by gel filtration and anion exchange chromatography. Among the bark maple polysaccharides, this study was focused on one of the neutral polysaccharides, inulin detected in maple syrup. Inulin has a well documented prebiotic activity. Monosaccharide compositions, linkage analyses, molecular weight and degree of crystallinity of the isolated polysaccharides were investigated by NMR, GC-MS, gel permeation chromatography (GPC) and X-ray diffraction. Biological studies will be performed on crude extract samples, sugar enriched samples and isolated complex polysaccharide.

Resumen [EN]

The sugar maple is a native species of northeastern North America and is widely appreciated and exploited for its sap, which is concentrated to produce maple syrup. However, its bark is not widely used, despite its content of a variety of bioactive compounds which could be applied in health products. Apart from polyphenols, higher molecular weight compounds such as oligo- and polysaccharides have been less explored so far. The aim of this investigation was to isolate and characterize a complex sugar from sugar maple bark and evaluate its biological activities. Two samples obtained by extraction with water and ethanol-water (v/v:1/1) at 80°C, have been studied in this research. Sugar components were concentrated by ethanol (95%) precipitation and ultrafiltration (5KDa MWCO) to obtain enriched polysaccharides fractions. The latter were separated by gel filtration and anion exchange chromatography. Among the bark maple polysaccharides, this study was focused on one of the neutral polysaccharides, inulin detected in maple syrup. Inulin has a well documented prebiotic activity. Monosaccharide compositions, linkage analyses, molecular weight and degree of crystallinity of the isolated polysaccharides were investigated by NMR, GC-MS, gel permeation chromatography (GPC) and X-ray diffraction. Biological studies will be performed on crude extract samples, sugar enriched samples and isolated complex polysaccharide.

Sobre el ponente

Tatjana Stevanovic Janezic

Dr. Tatjana Stevanovic Janezic

Wood chemistry Laboratory, Department of Wood and Forest Sciences, Université Laval, Québec City Flag of Canadá
Información Práctica
Ponencia
English (US)
junio 26, 2019 9:44 a. m.
20 minutos
Restaurante "El Tesico"
Autores
Tatjana stevanovic
Dr. Tatjana Stevanovic Janezic
Pierre b. kasanganaa
Palabras clave
gel filtration
ion-exchange chromatography
sugar maple bark
ultrafiltration
water and ethanol- water extracts