7th International Symposium of Pharmaceutical Sciences "VII SICF" -7th Symposium "Design, Production and Development of Drugs"

7th International Symposium of Pharmaceutical Sciences

VII SICF

CONFORMATIONAL CHARACTERIZATION OF IL-2 MUTEIN BY CIRCULAR DICHROISM AND FLORESCENCE: ITS UTILITY FOR THE INDUSTRY

Abstract

Circular dichroism and fluorescence spectroscopy are used in the biopharmaceutical industry to study the effects of manufacturing, storage and comparability conditions on protein conformation and stability. These results are often included in the regulatory files. At the Center of Molecular Immunology, a Cuban biotechnology industry, these methodologies have allowed the characterization of the conformational features of products under development, such as mIL2, a mutant of interleukin-2 for the treatment of melanoma and renal cancer. This product has 165 amino acids and a molecular weight of 18.3 kDa. Different batches of mIL2 were included to assess the consistency of the productive process. All lots showed similar features of secondary structure as determined by far-ultraviolet circular dichroism spectroscopy. The bands in the near ultraviolet circular dichroism spectra, which reflect the aromatic residues absorption suggest different three-dimensional folding. Besides, these lots showed different position of the emission maxima and spectral profile by fluorescence, upon selective excitation of Trp residues and Trp/Tyr. For this reason, the differences observed for batches of final product and active pharmaceutical ingredient in this process have an impact on their biological activity. Here we discuss the relationship between the spectroscopic response in different situations and biological activity. The evaluation of the conformational characteristics of proteins by orthogonal methodologies widely described in the state of the art: circular dichroism and fluorescence, and their possible impact on biological function is very useful for the biotechnology industry.

Resumen

Circular dichroism and fluorescence spectroscopy are used in the biopharmaceutical industry to study the effects of manufacturing, storage and comparability conditions on protein conformation and stability. These results are often included in the regulatory files. At the Center of Molecular Immunology, a Cuban biotechnology industry, these methodologies have allowed the characterization of the conformational features of products under development, such as mIL2, a mutant of interleukin-2 for the treatment of melanoma and renal cancer. This product has 165 amino acids and a molecular weight of 18.3 kDa. Different batches of mIL2 were included to assess the consistency of the productive process. All lots showed similar features of secondary structure as determined by far-ultraviolet circular dichroism spectroscopy. The bands in the near ultraviolet circular dichroism spectra, which reflect the aromatic residues absorption suggest different three-dimensional folding. Besides, these lots showed different position of the emission maxima and spectral profile by fluorescence, upon selective excitation of Trp residues and Trp/Tyr. For this reason, the differences observed for batches of final product and active pharmaceutical ingredient in this process have an impact on their biological activity. Here we discuss the relationship between the spectroscopic response in different situations and biological activity. The evaluation of the conformational characteristics of proteins by orthogonal methodologies widely described in the state of the art: circular dichroism and fluorescence, and their possible impact on biological function is very useful for the biotechnology industry.

About The Speaker

Leina Moro Pérez

Lic. Leina Moro Pérez

Centro de inmunología Molecular Flag of Cuba
Practical Info
No Presencial
English (US)
Not defined
30 minutes
Not defined
Authors
Msc. Sum Lai Lozada Chang
Lic. Leina Moro Pérez
Keywords
circular dichroism
fluorescence
mil2 and conformational characterization