VIII International Symposium on Chemistry and Pharmaceutical Sciences
SIPN
4th International Symposium "Chemistry of natural products”
Abstract
Agave brittoniana brachypus presents among its secondary metabolites steroidal saponins, of which several pharmacological activities are known, but studies concerning its toxicity are insufficient. Therefore, the objective of this research was to evaluate the oral toxicity of a crude of saponin from this plant at repeated doses in Wistar rats. Five experimental groups of ten animals (five per sex) were set up: a control group and four groups treated with doses of 50, 100 and 200 mg/kg of crude for 28 days. Daily clinical observations, physical examination, and weekly weighing were performed, as well as hematological and biochemical analysis at 0, 14, and 28 days. At the end of the period, the animals were sacrificed, autopsied, macroscopic pathology study was performed, and various organs were weighed. The trial concluded with 100% survival and no alterations were reported in the clinical signs evaluated. An increase in body weight was observed in all groups, corresponding to the growth curve of the species and line. All hematological and biochemical indicators remained within the normal parameters of the species. Macroscopically, a slight paleness was observed in the liver of some animals, but it was not considered of interest due to its low frequency of ocurrence and its presence in the control group; in adition, no significant differences were found in the relative weight of the organs analyzed. In conclusion, the crude of saponins from Agave brittoniana brachypus, at the administered doses and the experimental conditions, do not show toxic effects in Wistar rats.
Resumen
Agave brittoniana subps. brachypus presenta entre sus metabolitos secundarios saponinas esteroidales, de las cuales se conocen varias actividades farmacológicas, pero son insuficientes los estudios referentes a su toxicidad. El objetivo de la investigación fue determinar la toxicidad oral de un crudo de saponinas de dicha planta a dosis repetidas en ratas de la línea Wistar. Se confeccionaron cinco grupos experimentales de diez animales (cinco por sexo): un grupo control y cuatro grupos tratados con dosis de 50, 100 y 200 mg/kg del crudo durante 28 días. Se realizaron observaciones clínicas diarias, examen físico y pesaje semanal, así como análisis hematológico y bioquímico a los 0, 14 y 28 días. Cumplido el tiempo, se sacrificaron los animales, se les realizó autopsia, estudio anatomopatológico macroscópico y se pesaron varios órganos. El ensayo concluyó con un 100% de supervivencia y no se reportaron alteraciones en los signos clínicos evaluados. Se observó un incremento del peso corporal en todos los grupos, correspondiéndose con la curva de crecimiento de la especie y línea. Todos los indicadores hematológicos y bioquímicos se mantuvieron dentro de los parámetros normales de la especie. Macroscópicamente, no se observaron alteraciones de interés en los órganos analizados, y tampoco diferencias significativas en el peso relativo de estos. En conclusión, el crudo de saponinas de Agave brittoniana subps. brachypus, a las dosis administradas y las condiciones experimentales, no evidencia efectos tóxicos en ratas de la línea Wistar.
About The Speaker
Anna Elizabeth Cárdenas González
Discussion
