Development of a new purification process to obtain the IL-2 mutein

• Sum Lai Lozada

• Póster digital
• Spanish / Español

• interleukine 2
• muteín
• purification
• solubilization
• ultrafiltration

At the Molecular Immunology Center, was designed a mutant of the interleukin-2 molecule without interaction with the alpha chain of the IL-2 receptor. This new mutant shows higher antitumor activity and less toxicity than human IL-2, that´s why it is a better candidate for human cancer therapy. The mutein synthetic gene was cloned into the pET28a expression vector and protein is produced in the E. coli BL-21 (DE3) strain fused to a six histidine sequence at N terminal. Similar to other experienc...