Trabajos

QUINOLONYL DERIVATIVES AS HIV RNaseH INHIBITORS

  • Prof. Roberta Costi
  • 25/06/2019 10:47

The introduction of HAART for treating acquired immunodeficiency syndrome (AIDS) allowed reaching remarkable milestones, turning AIDS into a chronic disease. However, HAART still has several drawbacks, underscoring the demand of new antiretroviral agents with higher genetic barrier. Development of compounds able to inhibit essential but unexplored targets is mostly attractive to reduce selection of drug resistant strains, as reverse transcriptase (RT)-associated ribonuclease H (RH) function. Alt...

IDENTIFYING THE INTERACTIONS OF THREE IMMUNOSUPPRESSANTS WITH FKBP12 INTRAMOLECULAR RECEPTOR: A THEORETICAL STUDY

  • Sr. Anthuan Ferino Pérez

Understanding principles of molecular recognition is a challenging problem in contemporary molecular biology and theoretical biochemistry that could be approached with molecular modeling. Peptidyl-prolyl cis-trans isomerase FKBP1A (known as FKBP12) is a member of the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking and that binds the immunosuppressants rapamycin (sirolimus), everolimus and temsirolimus. An ...

``IN VITRO`` BIOLOGICAL AND BIOPHARMACEUTICAL EVALUATION OF THE INCLUSION COMPLEXES OF THE ANTIFUNGAL COMPOUND 2-(2-NITROVINYL)FURAN WITH BETA- CYCLODEXTRIN DERIVATIVES

  • Dr. Vivian Ruz

Introduction 2-(2-nitrovinyl)furan has antifungal activity, but limited stability. Inclusion complexes with β-CD derivatives are alternatives to solve this problem. This work aimed to evaluate their influence on the drug release, permeability and cytotoxicity, intended for vaginal administration. Methods Freeze-dried complexes (FD ICs) were prepared using a reported method (Ruz et al., 2012). Dissolution studies of pure drug and complexes were carried out in USP dissolution apparatus I. Dissolut...

METAL OXIDE NANOPARTICLES FOR POTENTIAL APPLICATION IN CHOLESTEROL DETECTION

  • Fernando Bordallo León

Cardiovascular diseases are one of the leading causes of death worldwide. It has been demonstrated that the probability of suffering one of these illness is linked to high blood cholesterol levels. Therefore, the fast detection of this lipid would improve the clinical diagnosis of these diseases. A system obtained on a glass graphite electrode with metal oxide nanoparticle, cholesterol oxidase and Nafion®, as a potential biosensor for the rapid detection of cholesterol, it was developing on this...

EFFECTS IN VITRO AND IN VIVO OF STII: A NATURAL COMPOUND WITH ANTITUMORAL PROPERTIES

  • Carmen de los Ángeles Soto Febles

SticholysinII (StII) is a protein purified from the sea anemone Stichodactyla helianthus. Based on its ability to form pores in membranes, we evaluated their effects in vitro and in vivo in tumor cells in order to study the antitumor properties of this toxin. In order to understand the cell pathways triggered upon membrane destabilization by StII, we have examined the mechanisms that mediate its cytotoxicity on tumor cells. StII's action on L1210, Raji, X63, B16, A549 tumor cells leads to an inc...

Identification of a potent inhibitor of LAPTc, an M17 aminopeptidase of Trypanosoma cruzi

  • Lic. Mirtha Elisa Aguado Casas

A novel target in Chagas disease is the leucil-aminopeptidase M17 of Trypanosoma cruzi (LAPTc). The objective of this work was to identify a potent inhibitor of LAPTc. The optimized LAPTc gene was synthesized and cloned in the vector pET-19b for its expression in Escherichia coli. The genetic construction pET-19brLAPTc allows an soluble and active production of rLAPTc in E. coli BL21(DE3)pLysS, by induction during 20 h at 25ºC with IPTG 1 mM. A level of expression of 12.53 % of the total cellul...

DEVELOPMENT OF A STABLE BIOPHARMACEUTICAL FORMULATION FOR A MONOCLONAL ANTIBODY aHER2

  • MsC. Yaiko Saddan Hernández Terrero

The development of pharmaceutical formulations of monoclonal antibodies is of vital importance to achieve effective and safe drugs. Hence, many research groups have focused on using different screening strategies to optimize the formulation for each antibody under study. Within the excipients used to carry out the screening of formulation are carbohydrates, salts, surfactants and amino acids. In our work we focused specifically on a monoclonal antibody aHer2 for the treatment of breast cancer ty...

INFLUENCIA DEL MEDIO DE CULTIVO PARA LA OBTENCIÓN DE LA PROTEÍNA ESTREPTOQUINASA RECOMBINANTE

  • Ing. Marlen Herrera

Desde el año 1989 se produce en el Centro de Ingeniería Genética y Biotecnología (CIGB) la Estreptoquinasa recombinante (SKr) para su uso como agente trombolítico. En los últimos años en Cuba se han venido obteniendo resultados favorables en estudios clínicos con el empleo de esta molécula para el tratamiento de las hemorroides. Debido a la necesidad de contar con un proceso tecnológico que cumpla con los requerimientos regulatorios de las Buenas Prácticas de Manufactura (GMP) y a los planes de ...

HYDROXIL RADICAL SCAVENGER MECHANISMS BY 2,4-DI-TERTBUTYLPHENOL AND 2,6-DI-SECBUTYLPHENOL PHYTOCOMPOUNDS: A COMPUTATIONAL STUDY

  • Lic. Daniel Alpízar Pedraza

Among Phyllanthus orbicularis K phytocompounds, 2,4-di-terbutilfenol o el 2,6-di-secbutilfenol are proposed as potent mutagenicity inhibitors produced by hydrogen peroxide, in S. tiphimurium. This DNA protected capacity, against oxidative damage can be explained, theoretically, by several ways. To date, has not been reported neither molecular mechanics as support to the antimutagenic activity of this compounds. In the present work in silico modelation was performed in order to propose a chemistr...

Translating pore forming proteins-induced innate immune responses into memory CD8+ T cells for vaccine development

  • MsC. Anaixis Del Valle

Induction of CD8+ T cell responses against pathogens and cancers is an important goal of modern immunology. Notably, increased ratios of effector/central memory T cells (Tef)/(Tcm) have been described for highly efficacious vaccines. One current approach of translational interest is the use of liposomes encapsulating pore-forming proteins (PFP), such as Listeriolysin O (LLO), which are very effective at priming strong and sustained CD8+ T cell responses. We have been recently shown that Sticholy...

Antitumor effects of a humanized antibody specific for the neoantigen GM3(Neu5Gc) ganglioside against Cmah-transfected cancer cells.

  • MsC. Denise Dorvignit Pedroso

Expressed on human tumors, the GM3(Neu5Gc) ganglioside represents a promising target for cancer immunotherapy. In absence of functional CMAH in humans, an enzyme required for Neu5Gc sialic acid biosynthesis, tumor-associated Neu5Gc expression has been linked to dietary uptake and metabolic incorporation. The use of cell lines for testing GM3(Neu5Gc)-targeting strategies is complicated by the complexity of sialic acid biology and the influence of culture conditions. Here, quantitative flow cytome...

CONFORMATIONAL CHARACTERIZATION OF IL-2 MUTEIN BY CIRCULAR DICHROISM AND FLORESCENCE: ITS UTILITY FOR THE INDUSTRY

  • Lic. Leina Moro Pérez

Circular dichroism and fluorescence spectroscopy are used in the biopharmaceutical industry to study the effects of manufacturing, storage and comparability conditions on protein conformation and stability. These results are often included in the regulatory files. At the Center of Molecular Immunology, a Cuban biotechnology industry, these methodologies have allowed the characterization of the conformational features of products under development, such as mIL2, a mutant of interleukin-2 for the ...

ZnO AND IRON OXIDES NANOPARTICLES WITH POTENTIAL APPLICATIONS IN BIOMEDICINE

  • Prof. Alicia M. Díaz García

ZnO and iron oxide (ZnONPs and IONPs) received a great attention due to the possibility to be used in several areas of chemistry, physics and material science. The study related with nanoparticle−biological system interactions has increased in the past decade. For biomedical applications, a protective layer is formed on the surface of the particles via specific interactions or chemical bonding between the active sites of NPs and the coating molecules. The surface coating determines the propertie...

High-Throughput Screen to Identify LAPTc Inhibitors as in vitro Antichagasic Agents Using RapidFire Mass Spectrometry

  • Lic. Mirtha Elisa Aguado Casas

The American trypanosomiasis better known as Chagas disease is a systemic infection caused by the protozoan Trypanosoma cruzi. It is estimated 56,000 new cases and 12,000 deaths annually in the Americas region in 21 countries. The chemotherapy of the disease is still inadequate. Several proteases involving in vital processes for the survival of parasites such as invasion, migration, nutrition, immune response evasion and immunomodulation have been described. The presence of the leucyl aminopepti...

POPULATION PHARMACOKINETIC/PHARMACODYNAMIC OF TWO NOVEL PEGYLATED rHuEPO

  • Dr. Gledys Reynaldo Fernández

Background and Objectives: Marketed formulations ior®EPOCIM, MIRCERA® and two new branched PEGylated erythropoietin formulations (32kDa-PEG2-rHuEPO and 40kDa-PEG2-rHuEPO) were administered a single dose by intravenous bolus in New Zealand rabbits. The aim of this work was to develop a population pharmacokinetic/pharmacodynamic (PK/PD) model describing in a simultaneous and integrated form the time course of erythropoiesis stimulation after erythropoietin administration. Methods: The PK/PD datase...

Design of a culture medium for the growth of the nematode control strain CIGBc8

  • Ing. Dayana Morales Borrell

The composition of a culture medium is one of the most important parameters to be analyzed in biotechnological processes with industrial purposes, because the growth medium represents more than one third of the production costs. The main aim of this study was to design an optimal culture medium for the growth of the nematode control strain CIGBc8. Mixture statistical experimental design was applied using the Design-Expert 10.0 Software to optimize the growth medium. The effect of different sourc...

Development of a nanotechnological system of antigens delivery to the intestinal mucosa and its evaluation for the use as a vaccine in the hidatidosis treatment

  • MsC. María Cecilia Silvarrey

Hydatid disease caused by Echinococcus granulosus (Eg), is one of the most important parasitic zoonosis in Uruguay, Argentina, Brasil, Chile and Perú. Economic losses in the cattle industry have been estimated at more than two billion dollars annually. Despite the socio-economic impact, hydatid disease remains a neglected zoonotic disease. In the last 5 decades, it has tried the generation of vaccines, obtaining little success. Tests on mice conducted by our research group, using recombinant Ech...

Performance characteristics of a fast real-time PCR assay for HBV DNA quantification

  • MsC. Anny Armas Cayarga

Detection of hepatitis B virus (HBV) DNA is particularly important for detection of early acute and of occult HBV infection. On the other hand, HBV DNA detection and quantification are essential to diagnose and treat chronic HBV infection. In this study, we evaluated the performance of the real-time PCR SUMASIGNAL VHB (un paso) (Immunoassay Center, Cuba). The clinical and analytical specificity of the assay was 100%. Intra-assay and interassay coefficients of variation ranged from 0.50 to 2.53% ...

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